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Category:Video game dataChronic ethanol ingestion suppresses thymocyte apoptosis in an animal model of alcoholic liver disease.
Chronic ethanol ingestion is known to induce oxidative stress and to cause liver damage. Our recent studies have shown that oxidative stress, generated by ethanol and leading to reactive oxygen species (ROS), is involved in mediating the ethanol-induced apoptosis in liver and thymocytes. In this study, we investigated whether ethanol-induced oxidative stress can cause the suppression of apoptosis in thymocytes. Ethanol-induced oxidative stress was examined by measuring lipid peroxide levels in thymocytes and observing thymic morphology. Thymocyte apoptosis was quantitatively determined using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. The results showed that ethanol treatment increased lipid peroxide levels in thymocytes; however, less apoptotic cells were detected after ethanol treatment. To evaluate the mechanism of ethanol-induced reduction of apoptosis in thymocytes, the effects of N-acetylcysteine (NAC), an antioxidant, were determined. When thymocytes were pretreated with NAC, an ethanol-induced reduction in apoptosis was reversed. Thus, our results provide evidence that ethanol-induced apoptosis was inhibited by NAC and that ROS may play a role in mediating ethanol’s apoptotic effects on thymocytes.Additional evidence that the delay in skin carcinogenesis by exposure to 7,12-dimethylbenz(a)anthracene in the nude mouse is related to an enhanced expression of the hypoxia-inducible factor-1alpha gene.
Exposure to 7,12-dimethylbenz(a)anthracene (DMBA) results in a delay in skin carcinogenesis and is associated with a selective increase in the number of HIF-1alpha-positive cells. We now show that the time course of the increase in DMBA-induced expression of HIF-1alpha mRNA in mouse skin is related to the time-course of the delay in skin tumor development. The results of qRT-PCR demonstrated that the increase in HIF-1alpha mRNA peaked at approximately 6 months after DMBA treatment
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