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Thursday, May 11, 2017

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If you enjoy my work, please support me on Patreon. Thank you!Dietary supplementation with a low fermentable dietary fibre preparation induces DNA hypermethylation of p16 promoter in human colorectal cancer cells.
Modification of promoter methylation status is a frequent event in tumourigenesis and the development of colorectal cancer. We therefore examined whether different fibre preparations have an impact on promoter methylation in colorectal cancer (CRC) cells. In HT29 CRC cells, DMH, a low fermentable dietary fibre preparation, reduced c-MYC transcription in a dose-dependent manner. In addition, DMH increased at least 21-fold the expression of p16 (CDKN2A) as compared to controls. Immunocytochemistry showed that p16 protein expression was markedly induced by DMH in CRC cells. Next, the methylation status of the p16 promoter was examined using methylation-specific PCR. DMH increased the methylation status of the p16 promoter. This DMH-induced methylation was abolished by co-incubation with the anti-
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