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During the past 15 years, pig skin has also been used for on-site production of heparin, epidermal growth factor (EGF) and tissue-type plasminogen activator (tPA) \[[@B34-sensors-18-04078],[@B35-sensors-18-04078],[@B36-sensors-18-04078]\].

[Table 1](#sensors-18-04078-t001){ref-type=”table”} shows the performances of the biosensors reported in the literature that used patches of pig skin as membranes.

The miniaturized biosensors that were described during the last years have been covered by one paper \[[@B37-sensors-18-04078]\]. In this review, it has been included three sensors using a carbon paste electrode with a working or counter electrode made of silicon, plastic or thermoplastics ([Figure 4](#sensors-18-04078-f004){ref-type=”fig”}).

2.4. Limitations and Scope of this Review {#sec2dot4-sensors-18-04078}
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The knowledge of microfluidics, chemical sensors and, more importantly, conductive tracks printed on biosensors, as well as their operation and characterization, is important for the design and interpretation of electrical biosensors. For this reason, the main focus of the present paper has been placed on the studies that reported the development of miniaturized biosensors. Consequently, the details of the experimental procedures for the fabrication of nanofabricated electrodes and the instrumental characterization of nanoscaled electrodes have been excluded.

In this regard, a review of the biological properties of metals that have been used as electrodes for biosensors would be of interest for designing novel miniaturized biosensors. Indeed, adhesion between the biological component and the electrode surface is particularly important. However, this area is far from being considered in the current literature, and further studies are thus required.

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https://desifaceup.in/upload/files/2022/06/KwCZiC9VA1s2WmQ5wRam_17_fce1a085f40c3d9522c4eb829acf569d_file.pdf
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